mouse lif duoset kit  (R&D Systems)

Journal: Frontiers in Plant Science

Article Title: CRISPR/Cas9 and Transgene Verification of Gene Involvement in Unfolded Protein Response and Recombinant Protein Production in Barley Grain

doi: 10.3389/fpls.2021.755788

Figure Lengend Snippet: Mouse leukemia inhibitory factor (mLIF) concentration based on ELISA assay in mature grain flour. Golden Promise (white) was used as a negative control. ORF mLIF was used as positive control (light gray). Crossed plants with overexpression (dark gray), crossed plants with knockout mutation (diagonal) and crossed plants with no mutation (dotted). Plants were grown in growth chamber or greenhouse. Error bars represents the standard error and significant differences by Student’s t -test. ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001.

Article Snippet: The Sandwhich ELISA assay of mLIF was done with a mouse LIF DuoSet ELISA kit from R&D Systems as described by the manufacturer.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Negative Control, Positive Control, Over Expression, Knock-Out, Mutagenesis

Journal: Frontiers in Plant Science

Article Title: CRISPR/Cas9 and Transgene Verification of Gene Involvement in Unfolded Protein Response and Recombinant Protein Production in Barley Grain

doi: 10.3389/fpls.2021.755788

Figure Lengend Snippet: Epidermal growth factor (EGF) concentration based on ELISA assay in mature grain flour. Golden Promise was used as a negative control. Controls (white), ORF EGF positive control (light gray) was grown in growth chamber and greenhouse. Crossed plants with overexpression (dark gray) and crossed plants with knockout mutation (diagonal). Error bars represents standard error. Significant changes found by Student’s t -test ( ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001).

Article Snippet: The Sandwhich ELISA assay of mLIF was done with a mouse LIF DuoSet ELISA kit from R&D Systems as described by the manufacturer.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Negative Control, Positive Control, Over Expression, Knock-Out, Mutagenesis